Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells

Summary Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the UK, with over 200,000 positive diagnoses annually. The infection is thought to cause reproductive complications including problems in conceiving a pregnancy through to miscarriage and early or stillbirth. One potential reason Chlamydia may impact upon pregnancy is through disrupting the embryo implantation at the earliest stages of pregnancy is by altering the ability of specific cells that line the uterus called stromal cells to respond to the hormone progesterone, the hormone responsible for preparing the uterus for pregnancy. The results of this study showed that Chlamydial infection of these uterus lining stromal cells decreased the levels of specific progesterone sensitive markers which are associated with early embryo implantation, suggesting a loss of responsiveness to progesterone treatment. These changes were accompanied by a decrease in the levels of RNA for the progesterone receptor which is responsible for progesterone activity, suggesting that this is a potential mechanism through which Chlamydia could directly inhibit the effects of progesterone on uterine cells.

Chlamydia trachomatis (Ct) infection has significant longterm reproductive consequences, leading to a range of complications including infertility, miscarriage and preterm birth (Tang et al. 2020).While the mechanisms through which Ct leads to adverse pregnancy outcomes remain unknown, one potential cause is via disruption of uterine-embryo implantation.Successful implantation requires endometrial stromal cells (ESC) differentiation into secretory cells, a progesterone-driven process known as decidualisation.Chlamydial infection can reverse this process in primary ESCs (Giakoumelou et al. 2017).This study aimed to investigate potential mechanisms underpinning this process.SHT290-cells (Telomerase-immortalised ESC line) were decidualised for 96 hours using a previously established protocol (Giakoumelou et al. 2017).Cells were exposed to Ct (serovar E) at a multiplicity of infection (MOI) 0.1, 1 or mock infection consisting of control media for 2 h before replacement with fresh decidualisation media (Giakoumelou et al. 2017).Chlamydial infection was quantified by qPCR, decidualisation was assessed by changes in cell morphology (for details see Supplementary file 1, see section on supplementary materials given at the end of the article) and by alterations in gene expression.48h post-infection, RNA was isolated (RNeasy mini kit, Qiagen) as per manufacturer's instructions.RNA samples were treated using DNAse 1 (Sigma Aldrich) before RT (Precision RT Premix, Primerdesign, UK).Quantitative real-time PCR was performed using 40ng of cDNA in a total volume of 20μl using Precision 2X qMasterMix with SYBR Green (PrimerDesign).Samples were analysed using 2−(ΔΔCt) quantification method relative to a mixed (pooled cDNA from each treatment) reference sample, using the geometric mean of stable housekeeping genes GAPDH and β-actin (Target and housekeeper primer sequences-Supplementary file 1).Ct (MOI1) exposure was accompanied by a marked decrease in Prolactin mRNA (P < 0.05) and the endometrial receptivity marker SPP-1 (P = 0.04), an essential implantation and receptivity mediator which is known to be expressed in primary ESC and during normal cycles within the endometrium (Marshall et al. 2011).Progesterone receptor mRNA expression was similarly reduced (P < 0.05) in Ct infected cells, conversely pro-inflammatory COX-2 mRNA expression was robustly elevated (P =0.03) compared to noninfected cells.Testosterone is a likely key determinant of primary ESC decidualisation (Gibson et al. 2016) the effects of chlamydial infection on the expression of the steroid biosynthesis markers CYP17A1 and AKR1C3 was investigated.CYP17A1 and AKR1C3 were significantly downregulated by (P =0.02 and P =0.03, respectively) by MOI1 compared to the non-decidualised control (summarised in Fig. 1).
Findings of this study may suggest a possible pathway through which Ct may dysregulate endometrial decidualisation.
Steroid hormones, particularly progesterone, are key drivers of ESC decidualisation.Progesterone removal results in a reversion of decidual phenotype characterised by changes in cellular morphology and reduced expression of prolactin, a key factor in the process of decidualisation (Evans & Salamonsen 2014), similar to the changes observed with Ct infection in the current study and in primary ESC (Giakoumelou et al. 2017).We suggest reduced progesterone receptor expression may dampen normal progesterone action in Ct infection.Intracrine biosynthesis of endometrial androgens during decidualisation has also been suggested to be a key driver of ESC decidualisation (Gibson et al. 2016).The results of the current study, demonstrating that infection reduces expression of androgen biosynthetic enzymes suggests that Ct also inhibits the ability of ESC to synthesise testosterone from progesterone.In addition to direct effects on steroid pathways, elevation in COX-2 expression may likely lead to an altered local prostanoid profile which may, in turn, promote a pro-inflammatory tone within uterine tissues.
This study has identified potential mechanisms through the progesterone pathway by which Ct may influence the responsiveness of ESCs to progesterone, negatively impacting upon implantation and successful pregnancy.Recognising that there may be other pathways through which Ct may impact implantation, more in-depth investigations are needed to determine the clinical significance of our findings.

Supplementary materials
This is linked to the online version of the paper at https://doi.org/10.1530/RAF-20-0069.

Declaration of interest
Nick Wheelhouse is an Associate Editor of Reproduction and Fertility.Nick Wheelhouse was not involved in the review or editorial process for this paper, on which he is listed as an author.

Funding
This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.