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Iman Al-Saleh Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Serdar Coskun Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Reem Al-Rouqi Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Tahreer Al-Rajudi Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Chafica Eltabache Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Mai Abduljabbar Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Saad Al-Hassan Reproductive Medicine Unit, Department of Obstetrics and Gynecology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

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Investigation 36 1117 – 1120 . ( https://doi.org/10.3275/9097 ) Atig F Kerkeni A Saad A Ajina M 2017 Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men . Journal of Assisted

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Mathias Abiodun Emokpae Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria

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Somieye Imaobong Brown Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria

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aging on increase DNA fragmentation was reported to be a contributing factor to the low success rate of assisted reproduction technology (ART) treatment in infertile couples ( Alvarez Sedó et al. 2017 ). Individuals should be counseled to start a

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Shen Chuen Khaw NHS Tayside, Ninewells Hospital, Dundee, UK

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Zhen Zhe Wong International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Federal Territory of Kuala Lumpur, Malaysia

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Richard Anderson MRC Centre for Reproductive Health, Queen’s Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK

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Sarah Martins da Silva Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK

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the production of malondialdehyde (MDA) and 4-hydroxynonenal (4HNE), which oxidises the lipid membrane and causes fragmentation of both nuclear and mitochondrial DNA in sperm ( de Lamirande & Gagnon 1992 , Kodama et al. 1997 , Gomez et al. 1998

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