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Jason R Herrick Omaha’s Henry Doorly Zoo and Aquarium, Omaha, Nebrask, USA
Colorado Center for Reproductive Medicine, Lone Tree, Colorado, USA

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Cayla J Iske Department of Animal Science, Iowa State University, Ames, Iowa, USA

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Rachel M Santymire Davee Center for Epidemiology and Endocrinology, Lincoln Park Zoo, Chicago, Illinois, USA

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Colleen Lynch Riverbanks Zoo and Garden, Columbia, South Carolina, USA

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Mattina Alonge Davee Center for Epidemiology and Endocrinology, Lincoln Park Zoo, Chicago, Illinois, USA

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Rebecca L Krisher Colorado Center for Reproductive Medicine, Lone Tree, Colorado, USA

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Cheryl L Morris Omaha’s Henry Doorly Zoo and Aquarium, Omaha, Nebrask, USA
Department of Animal Science, Iowa State University, Ames, Iowa, USA

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. Materials and methods Animals Male snow leopards in this study were included in the Snow Leopard SSP and maintained in USDA and/or AZA-accredited facilities. All procedures were reviewed and approved by the Animal Care and Use Committees of each

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Jennifer B Nagashima Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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Marcia de Almeida Monteiro Melo Ferraz Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Gene Center, Ludwig-Maximilians University, Munich, Bayern, Germany

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Sarah H Kamen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Department of Biological and Environmental Sciences, Longwood University, Farmville, Virginia, USA

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Nucharin Songsasen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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USFWS guidelines and Smithsonian Institution International Animal Care and Use Committee approval (IACUC #18-05). Sperm collection and processing A single collection attempt was made per animal in the 2018 and 2019 breeding seasons (January

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Madeleine L H Campbell Department of Pathobiology and Population Sciences, The Royal Veterinary College, Hawkshead Lane, South Mymms, UK

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Introduction We live, as the programme for the recent Fertility 2021 conference evidenced, in an extraordinary age where assisted reproductive techniques (ARTs) enable us not only to freeze gametes for future use, or to perform fertilisation

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Janine Meuffels-Barkas Cryovault, Rhino Force SA NPC, Tokai, South Africa
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa

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Sandra Wilsher The Paul Mellon Laboratory, Brunswick, Newmarket, Suffolk, UK

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W R Twink Allen The Paul Mellon Laboratory, Brunswick, Newmarket, Suffolk, UK

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Cyrillus Ververs VetRepSol, Rhenen, Utrechtsestraatweg, TW, The Netherlands

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Imke Lueders Cryovault, Rhino Force SA NPC, Tokai, South Africa
Mammal Research Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Hatfield, South Africa

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difficulties. For example, genetically diverse mating partners may be located at different facilities in other countries or continents, and fertility may be sub-optimal when the social needs of captive animals are not met. The use of assisted reproductive

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