Filter

Refine by date

—

Refine by collections

  • Print
  • Save

Search Results

You are looking at 1 - 10 of 17 items for :

  • "DNA fragmentation" x
Clear All
Maria Handzhiyska Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Maria Handzhiyska in
Google Scholar
PubMed Close
,
Rumiana Ganeva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Rumiana Ganeva in
Google Scholar
PubMed Close
,
Dimitar Parvanov Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Dimitar Parvanov in
Google Scholar
PubMed Close
,
Margarita Ruseva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Margarita Ruseva in
Google Scholar
PubMed Close
,
Petar Eftimov Department of Cell and Developmental Biology, Faculty of Biology, SU St. Climent Ohridski, Sofia, Bulgaria

Search for other papers by Petar Eftimov in
Google Scholar
PubMed Close
,
Vilyana Georgieva Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Vilyana Georgieva in
Google Scholar
PubMed Close
,
Denitsa Velikova Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Denitsa Velikova in
Google Scholar
PubMed Close
, and
Georgi Stamenov Department of Obstetrics and Gynecology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Georgi Stamenov in
Google Scholar
PubMed Close

). The selection of human spermatozoa with better motility, a lower number of morphological abnormalities, and decreased levels of DNA fragmentation has been proven to be useful for improving fertilization, implantation, and ongoing pregnancy ( Lundin et

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0052
Volume/Issue:
Volume 5: Issue 2
Open access
Rumiana Ganeva Research Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Rumiana Ganeva in
Google Scholar
PubMed Close
,
Dimitar Parvanov Research Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Dimitar Parvanov in
Google Scholar
PubMed Close
,
Denitsa Velikova Andrology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Denitsa Velikova in
Google Scholar
PubMed Close
,
Magdalena Vasileva Embryology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Magdalena Vasileva in
Google Scholar
PubMed Close
,
Kristina Nikolova Embryology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Kristina Nikolova in
Google Scholar
PubMed Close
, and
Georgi Stamenov Obsterics and Gynecology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

Search for other papers by Georgi Stamenov in
Google Scholar
PubMed Close

characteristics have emerged as important markers of sperm quality, such as sperm DNA fragmentation (SDF) ( Jin et al. 2015 , Chen et al. 2020 ) and the sperm zona-adhesion ability ( Hamada et al. 2012 , Ganeva et al. 2019 a ). Numerous studies have

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0041
Volume/Issue:
Volume 2: Issue 3
Open access
Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Alena J Hungerford in
Google Scholar
PubMed Close
,
Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

Search for other papers by Hassan W Bakos in
Google Scholar
PubMed Close
, and
Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Robert J Aitken in
Google Scholar
PubMed Close

& Sakkas 2019 ). The quality of the isolated cells was monitored by reference to sperm motility, vitality, morphology, mitochondrial ROS production, lipid aldehyde generation, and DNA fragmentation. The results emphasize the unique ability of the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-22-0133
Volume/Issue:
Volume 4: Issue 2
Open access
Fabien Joao Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Fabien Joao in
Google Scholar
PubMed Close
,
Cyntia Duval Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Cyntia Duval in
Google Scholar
PubMed Close
,
Marie-Claire Bélanger Fertilys Fertility Center, Laval, Quebec, Canada
Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), CHUM Research Center, Montreal, Quebec, Canada

Search for other papers by Marie-Claire Bélanger in
Google Scholar
PubMed Close
,
Julie Lamoureux Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Julie Lamoureux in
Google Scholar
PubMed Close
,
Cheng Wei Xiao Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Cheng Wei Xiao in
Google Scholar
PubMed Close
,
Senem Ates Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Senem Ates in
Google Scholar
PubMed Close
,
Moncef Benkhalifa Fertilys Fertility Center, Laval, Quebec, Canada
Université Picardie Jules Verne, Médecine et Biologie de la Reproduction et Laboratoire PERITOX, CBH-CHU Amiens Picardie, Amiens, France

Search for other papers by Moncef Benkhalifa in
Google Scholar
PubMed Close
, and
Pierre Miron Fertilys Fertility Center, Laval, Quebec, Canada

Search for other papers by Pierre Miron in
Google Scholar
PubMed Close

fluid oxidative stress was finally evaluated using the MiOXSYS. Sperm DNA fragmentation Sperm DNA fragmentation was measured using the In-Situ Cell Death Detection Kit (Roche) in 604 patients according to the manufacturer’s recommendations

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0005
Volume/Issue:
Volume 3: Issue 2
Open access
Mathias Abiodun Emokpae Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria

Search for other papers by Mathias Abiodun Emokpae in
Google Scholar
PubMed Close
and
Somieye Imaobong Brown Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria

Search for other papers by Somieye Imaobong Brown in
Google Scholar
PubMed Close

aging on increase DNA fragmentation was reported to be a contributing factor to the low success rate of assisted reproduction technology (ART) treatment in infertile couples ( Alvarez Sedó et al. 2017 ). Individuals should be counseled to start a

Article Type:
Review Article
Collections:
Clinical research and reviews
DOI:
https://doi.org/10.1530/RAF-20-0046
Volume/Issue:
Volume 2: Issue 1
Open access
Fatima Kazue Okada Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

Search for other papers by Fatima Kazue Okada in
Google Scholar
PubMed Close
,
Rhayza Roberta Andretta Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

Search for other papers by Rhayza Roberta Andretta in
Google Scholar
PubMed Close
, and
Deborah Montagnini Spaine Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

Search for other papers by Deborah Montagnini Spaine in
Google Scholar
PubMed Close

calculated. Evaluation of nuclear DNA integrity The nuclear DNA fragmentation was evaluated using the alkaline comet assay (Comet) ( Blumer et al. 2008 , Intasqui et al. 2016 ). The samples were diluted in 0.75% low melting point agarose in Tris

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-20-0018
Volume/Issue:
Volume 1: Issue 1
Open access
Ndivhuho B Takalani Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa

Search for other papers by Ndivhuho B Takalani in
Google Scholar
PubMed Close
,
Elizabeth M Monageng Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa

Search for other papers by Elizabeth M Monageng in
Google Scholar
PubMed Close
,
Kutullo Mohlala Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa

Search for other papers by Kutullo Mohlala in
Google Scholar
PubMed Close
,
Thomas K Monsees Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa

Search for other papers by Thomas K Monsees in
Google Scholar
PubMed Close
,
Ralf Henkel Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa
Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK
LogixX Pharma, Theale, Reading, Berkshire, UK

Search for other papers by Ralf Henkel in
Google Scholar
PubMed Close
, and
Chinyerum S Opuwari Department of Medical Biosciences, University of the Western Cape, Bellville, South Africa

Search for other papers by Chinyerum S Opuwari in
Google Scholar
PubMed Close

for MDA Khoubnasabjafari et al. (2015) DNA fragmentation TUNEL is a technique for identifying apoptotic DNA fragmentation that is frequently used to recognize and measure apoptotic cells or to find cells that have excessively

Article Type:
Review Article
DOI:
https://doi.org/10.1530/RAF-23-0024
Volume/Issue:
Volume 4: Issue 3
Open access
Leah Calvert School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Leah Calvert in
Google Scholar
PubMed Close
,
Jacinta H Martin School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Jacinta H Martin in
Google Scholar
PubMed Close
,
Amanda L Anderson School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Amanda L Anderson in
Google Scholar
PubMed Close
,
Ilana R Bernstein School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Ilana R Bernstein in
Google Scholar
PubMed Close
,
Nathan D Burke School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Nathan D Burke in
Google Scholar
PubMed Close
,
Geoffry N De Iuliis School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Geoffry N De Iuliis in
Google Scholar
PubMed Close
,
Andrew L Eamens School of Health, University of the Sunshine Coast, Maroochydore, QLD, Australia

Search for other papers by Andrew L Eamens in
Google Scholar
PubMed Close
,
Matthew D Dun Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia
Cancer Signalling Research Group, School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Matthew D Dun in
Google Scholar
PubMed Close
,
Brett D Turner Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW, Australia
Priority Research Centre for Geotechnical Science and Engineering, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Brett D Turner in
Google Scholar
PubMed Close
,
Shaun D Roman NSW Health Pathology, Newcastle, NSW, Australia

Search for other papers by Shaun D Roman in
Google Scholar
PubMed Close
,
Mark P Green School of BioSciences, Faculty of Science, University of Melbourne, Victoria, Australia

Search for other papers by Mark P Green in
Google Scholar
PubMed Close
, and
Brett Nixon School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia
Infertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton Heights, Newcastle, NSW, Australia

Search for other papers by Brett Nixon in
Google Scholar
PubMed Close

slides were then visualized and imaged on an AXIOplan Imager 2 fluorescence microscope. The degree of DNA fragmentation was determined using Comet Assay IV software (Perceptive Instruments, Suffolk, UK) in which the relative fluorescence intensity of the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0087
Volume/Issue:
Volume 5: Issue 1
Open access
Iman Al-Saleh Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Iman Al-Saleh in
Google Scholar
PubMed Close
,
Serdar Coskun Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Serdar Coskun in
Google Scholar
PubMed Close
,
Reem Al-Rouqi Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Reem Al-Rouqi in
Google Scholar
PubMed Close
,
Tahreer Al-Rajudi Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Tahreer Al-Rajudi in
Google Scholar
PubMed Close
,
Chafica Eltabache Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Chafica Eltabache in
Google Scholar
PubMed Close
,
Mai Abduljabbar Environmental Health Program, Research Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Mai Abduljabbar in
Google Scholar
PubMed Close
, and
Saad Al-Hassan Reproductive Medicine Unit, Department of Obstetrics and Gynecology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia

Search for other papers by Saad Al-Hassan in
Google Scholar
PubMed Close

Investigation 36 1117 – 1120 . ( https://doi.org/10.3275/9097 ) Atig F Kerkeni A Saad A Ajina M 2017 Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men . Journal of Assisted

Article Type:
Research Article
Collections:
Clinical research and reviews
DOI:
https://doi.org/10.1530/RAF-20-0062
Volume/Issue:
Volume 2: Issue 2
Open access
Maria Dri Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy

Search for other papers by Maria Dri in
Google Scholar
PubMed Close
,
Francesca Gioia Klinger Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy

Search for other papers by Francesca Gioia Klinger in
Google Scholar
PubMed Close
, and
Massimo De Felici Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy

Search for other papers by Massimo De Felici in
Google Scholar
PubMed Close

, stimulating at the same time granulosa cell proliferation and reducing DNA fragmentation in such cells through the PI3K/Akt pathway. In this regard, it is worthy to mention that in a model of mouse gestational diabetes, the PMF formation in the ovaries of a

Article Type:
Review Article
DOI:
https://doi.org/10.1530/RAF-21-0038
Volume/Issue:
Volume 2: Issue 3
Open access