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Maria Handzhiyska Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Rumiana Ganeva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Dimitar Parvanov Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Margarita Ruseva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Petar Eftimov Department of Cell and Developmental Biology, Faculty of Biology, SU St. Climent Ohridski, Sofia, Bulgaria

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Vilyana Georgieva Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Denitsa Velikova Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Georgi Stamenov Department of Obstetrics and Gynecology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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-mediated sperm binding ( Huszar et al. 2007 , West et al. 2022 ) and magnetic-activated cell sorting ( Nadalini et al. 2014 ). Another innovative sperm selection technique involving cumulus matrix (CM) penetration in a dish has recently been developed

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R Appeltant Nuffield Department of Women’s and Reproductive Health, Women’s Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK

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B V Adeniran Nuffield Department of Women’s and Reproductive Health, Women’s Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK

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S A Williams Nuffield Department of Women’s and Reproductive Health, Women’s Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK

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cumulus matrix, which plays a key role in in vivo fertilization ( Salustri et al . 2004 ). In the ovary, HA is to be found not only in the cumulus matrix ( Lo et al . 2019 ) but also in the theca cells and follicular fluid ( Christensen et al . 2015

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