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Scott C Mackenzie MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Catherine A Moakes Birmingham Clinical Trials Unit, University of Birmingham, Birmingham, UK

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W Colin Duncan MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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Stephen Tong Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Australia

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Andrew W Horne MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

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or ectopic pregnancy recurrence between management methods ( de Bennetot et al. 2012 , Fernandez et al. 2013 , Park et al. 2017 ). Approximately 30% of women with a scan-diagnosed ectopic pregnancy actively managed with methotrexate experience

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Charvi Kanodia Edinburgh Medical School, University of Edinburgh, UK

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Michael P Rimmer MRC Centre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, UK

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Kathleen Duffin Biomedical Sciences, University of Edinburgh, UK

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Rod T Mitchell MRC Centre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, UK

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Controlled clinical study To investigate the effect of methotrexate, paclitaxel, ifosfamide, and cisplatin on fertility in poor-risk nonseminomatous germ cell tumours Testosterone, FSH, LH, sperm analysis, fatherhood 30 30 The majority of men

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Bethany Chung Royal Infirmary of Edinburgh, Edinburgh, UK and MRC Centre for Reproductive Health

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Charlotte Greene Royal Infirmary of Edinburgh, Edinburgh, UK and MRC Centre for Reproductive Health

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Alice Pearson Royal Infirmary of Edinburgh, Edinburgh, UK and MRC Centre for Reproductive Health

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Lisa M Starrs Royal Infirmary of Edinburgh, Edinburgh, UK and MRC Centre for Reproductive Health

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W Colin Duncan Royal Infirmary of Edinburgh, Edinburgh, UK and MRC Centre for Reproductive Health
The University of Edinburgh, Edinburgh, UK

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.005* Number > 1 day admission 31 (32%) 15 (17%) 0.03* hCG, human chorionic gonadotrophin; MTX, methotrexate. *Represents statistically significant result ( P < 0.05). Analysis was done by Student t -test for parametric data and Mann

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McKenzie L J Haimon Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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Eliab Estrada-Cortés Department of Animal Sciences, University of Florida, Gainesville, Florida, USA
Campo Experimental Centro Altos de Jalisco, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Tepatitlán de Morelos, Jalisco, México

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Thiago Fernandes Amaral Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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Jeremy Block Department of Animal Science, University of Wyoming, Laramie, Wyoming, USA

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Surawich Jeensuk Department of Animal Sciences, University of Florida, Gainesville, Florida, USA
Department of Livestock Development, Bureau of Biotechnology in Livestock Production, Pathum Thani, Thailand

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Tatiane S Maia Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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Quinn A Hoorn, Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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Masroor Sagheer, Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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João H Bittar Department of Large Animal Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA

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Peter J Hansen Department of Animal Sciences, University of Florida, Gainesville, Florida, USA

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one-carbon metabolism are those driving the folate and methionine cycles. The importance of one-carbon metabolism for the preimplantation embryo is indicated by studies showing that the addition of the folate antagonist methotrexate blocked embryonic

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