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Maria Handzhiyska Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Rumiana Ganeva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Dimitar Parvanov Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Margarita Ruseva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Petar Eftimov Department of Cell and Developmental Biology, Faculty of Biology, SU St. Climent Ohridski, Sofia, Bulgaria

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Vilyana Georgieva Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Denitsa Velikova Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Georgi Stamenov Department of Obstetrics and Gynecology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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the CM membrane on sperm characteristics is still scarcely studied. The present study was designed to assess whether the introduced selection through CM may have a positive influence on sperm motility, morphology, and DNA fragmentation levels

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Steven Reynolds Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Sarah J Calvert Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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Stephen J Walters School of Health Related Research, University of Sheffield, Regent Court, Sheffield, UK

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Martyn N Paley Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Allan A Pacey Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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Introduction Asthenozoospermia is defined as <32% progressive sperm motility in semen, and while it can be readily identified at semen analysis, the underlying causes are not known ( WHO 2010 ). A number of studies have examined

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Shen Chuen Khaw NHS Tayside, Ninewells Hospital, Dundee, UK

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Zhen Zhe Wong International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Federal Territory of Kuala Lumpur, Malaysia

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Richard Anderson MRC Centre for Reproductive Health, Queen’s Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK

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Sarah Martins da Silva Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK

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. 2005 , Dokmeci 2005 , Adewoyin et al. 2017 ). In vitro , addition of carnitine to culture media increases sperm motility and vitality ( Tanphaichitr 1977 , Banihani et al. 2014 ). Notably, men with abnormal semen parameters have been reported to

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Lisa Windhofer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Auke Boersma Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Maik Dahlhoff Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Thomas Rülicke Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

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Kerstin E Auer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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the expansion of a line ( Behringer et al. 2014 , Taft 2017 ). In addition, sperm collection is performed to investigate sperm quality traits like sperm motility or swimming velocity. These traits can work as a proxy to estimate male fertility in

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Jennifer B Nagashima Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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Marcia de Almeida Monteiro Melo Ferraz Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Gene Center, Ludwig-Maximilians University, Munich, Bayern, Germany

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Sarah H Kamen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Department of Biological and Environmental Sciences, Longwood University, Farmville, Virginia, USA

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Nucharin Songsasen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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( Lockyear et al. 2009 ), and cryoprotectant and post-thaw culture temperatures ( Franklin et al. 2018 ), have improved survival and motility post-thaw. However, sperm motility/viability has been shown to drop precipitously during post-thaw incubation in

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Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

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Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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in a wide variety of ways, including reduced sperm number, lowered sperm motility, abnormal sperm morphology, impaired capacitation and sperm-egg recognition, lowered vitality, DNA damage, and oxidative stress ( Zini & Libman 2006 , Aitken & Roman

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Murong Xu Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Mingpeng Zhao Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Raymond Hang Wun Li Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China

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Zhixiu Lin Hong Kong Institute of Integrative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China

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Jacqueline Pui Wah Chung Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin Chiu Li Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin-Lap Lee School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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David Yiu Leung Chan Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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clinical trials studying the effects of N-9 focused on the sperm structure and functions. Sperm motility determines the ability of sperm to move toward the egg through the female reproductive tract. It is considered as the most important parameter to

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Fatima Kazue Okada Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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Rhayza Roberta Andretta Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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Deborah Montagnini Spaine Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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activity ( Shen et al. 2019 ), and acrosome integrity ( Levitas et al. 2006 , Shen et al. 2019 ). Mayorga-Torres et al. (2015) have shown that a longer EA is associated with increases in semen volume and sperm count. Sperm motility, viability

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Huidrom Yaiphaba Meitei Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal. Manipal Academy of Higher Education, Manipal, India

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Shubhashree Uppangala Division of Reproductive Genetics, Department of Reproductive Science, Kasturba Medical College, Manipal. Manipal Academy of Higher Education, Manipal, India

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Vani Lakshmi R Department of Data Science, Prasanna School of Public Health, Manipal, Manipal Academy of Higher Education, Manipal, India

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Guruprasad Kalthur Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India

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Satish Kumar Adiga Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal. Manipal Academy of Higher Education, Manipal, India

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motility, and morphological abnormalities were recorded. Sperm concentration was determined using a Makler counting chamber. Sperm motility was manually assessed in 200 spermatozoa on a slide using 40× objective under a compound microscope. Sperm vitality

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Ola S Davis Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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Vivien B Truong Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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Katie D Hickey Department of Research and Development, Semex Alliance, Guelph, Ontario, Canada

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Laura A Favetta Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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(in the case of ART use), or in the female reproductive tract ( Vitku et al. 2016 ). Sperm require both motility and capacitation abilities in order to fertilize an egg. In 2015, Lukacova et al. reported decreased bovine sperm motility after in

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