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al. 1997 , Kruger & Coetzee 1999 , Van Waart et al. 2001 , Jin et al. 2015 , Dcunha et al. 2022 ). One of the most commonly applied techniques for sperm selection is swim-up ( Volpes et al. 2016 ). Previous studies have proven that the
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devices ( Quinn et al. 2018 , Parrella et al. 2019 ), and motile sperm organelle morphology examination ( Bartoov et al. 2003 , Garolla et al. 2014 ). However, following the natural sperm selection process, another non-invasive approach has
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Monash IVF Group, Sydney, NSW, Australia
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, level of mitochondrial ROS generation, and DNA damage. Not surprisingly, all of the sperm selection techniques generated a reduced number of spermatozoa compared with the unselected control ( P <0.001). With respect to the individual sperm isolation
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, different authors have investigated the effect of sperm preparation before freezing as a potentially superior sperm selection approach. However, studies are conflicting and lack consistency especially in relation to: The number of pre-freeze and post
Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia
Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia
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SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom
School of Biological Sciences, The University of Adelaide, Adelaide, Australia
Department of Physics, College of Science, Yonsei University, Seoul, South Korea
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Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia
Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia
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the selection of sperm for intracytoplasmic sperm injection (ICSI), a process currently performed by the embryologist. The development of new assessment criteria for sperm selection might arise through the use of unsupervised AI, where new markers of
Faculty of Medicine and Life Sciences, Hasselt University, Agoralaan, Diepenbeek, Belgium
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Martinez Nuño V Flores-Saiffe Farias A Valencia-Murilloa R Drakeley A Garcia-Sandoval JP & Cohen J 2022 Computer software (SiD) assisted real-time single sperm selection associated with fertilization and blastocyst formation . Reproductive
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using hyaluronan-based sperm selection for intracytoplasmic sperm injection (ICSI) ( Miller et al. 2019 ). This example was prespecified, the number of tests performed was reasonably modest, and the corresponding test clearly indicated that the data