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Emmanuel Amabebe Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Steven Reynolds Academic Unit of Radiology, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK

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Dilly O C Anumba Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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The connection between vaginal microbiota-metabolite profiles and preterm delivery (PTD, birth <37 weeks’ gestation) has been a topical subject. Previous research has focused on specific metabolites using proton NMR spectroscopy ( 1 H-NMR

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Steven Reynolds Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Sarah J Calvert Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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Stephen J Walters School of Health Related Research, University of Sheffield, Regent Court, Sheffield, UK

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Martyn N Paley Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Allan A Pacey Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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and lower motility populations by 1 H-NMR ( Reynolds et al. 2017 a ). This showed that the integrals from both the choline and lactate/lipid regions of the NMR spectrum were significantly higher in sperm with poorer motility compared to those with

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Emmanuel Amabebe Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Noopur Bhatnagar Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Nitin Kamble School of Dentistry, University of Sheffield, Sheffield, UK

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Steven Reynolds Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK

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Dilly O Anumba Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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were determined by liquid chromatography-mass spectrometry and proton NMR spectroscopy ( 1 H-NMR). Figure 1 Lactobacillus crispatus inhibits growth of Gardnerella vaginalis . (A) Gram staining showing zone of inhibition (ZoI, Gram-positive rods

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Camila N Ortiz Department of Basic Sciences, Ponce Health Sciences University, Ponce Research Institute, Ponce, Puerto Rico, USA

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Annelyn Torres-Reverón DHR Health Institute for Research and Development, Edinburg, Texas, USA

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Caroline B Appleyard Department of Basic Sciences, Ponce Health Sciences University, Ponce Research Institute, Ponce, Puerto Rico, USA
Department of Internal Medicine, Ponce Health Sciences University, Ponce, Puerto Rico, USA

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transformation in primary and metastatic ovarian cancer . PLoS ONE 6 e19963. ( https://doi.org/10.1371/journal.pone.0019963 ) Ghazi N Arjmand M Akbari Z Mellat AO Saheb-Kashaf H Zamani Z 2016 1H NMR-based metabolomics approaches as non

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L B P M Stevens Brentjens Department of Obstetrics and Gynaecology, Maastricht University Medical Centre+, Maastricht, The Netherlands
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands

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D Obukhova GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands
Department of Clinical Genetics, Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands

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B Delvoux Department of Obstetrics and Gynaecology, Maastricht University Medical Centre+, Maastricht, The Netherlands
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands

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J E den Hartog Department of Obstetrics and Gynaecology, Maastricht University Medical Centre+, Maastricht, The Netherlands
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands

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B N Bui Department of Gynaecology & Reproductive Medicine, University Medical Centre Utrecht, Heidelberglaan, Utrecht, The Netherlands

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F Mol Centre for Reproductive Medicine, Reproduction and Development, Amsterdam University Medical Centre, University of Amsterdam, Meibergdreef, Amsterdam, The Netherlands

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J P de Bruin Department of Obstetrics and Gynaecology, Jeroen Bosch Hospital, Henri Dunantstraat, Hertogenbosch, The Netherlands

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D Besselink Department of Obstetrics and Gynaecology, Radboud University Medical Centre, Geert Grooteplein Zuid, Nijmegen, The Netherlands

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G Teklenburg Isala Fertility Clinic, Isala Hospital, Dokter van Heesweg, Zwolle, The Netherlands

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F Morgan Department of Complex Tissue Regeneration, MERLN Institute, Maastricht University, Maastricht, The Netherlands

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M Baker Department of Complex Tissue Regeneration, MERLN Institute, Maastricht University, Maastricht, The Netherlands

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F J M Broekmans Department of Gynaecology & Reproductive Medicine, University Medical Centre Utrecht, Heidelberglaan, Utrecht, The Netherlands

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R J T van Golde Department of Obstetrics and Gynaecology, Maastricht University Medical Centre+, Maastricht, The Netherlands
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands

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M Zamani Esteki GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands
Department of Clinical Genetics, Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands
Division of Obstetrics and Gynaecology, Department of Clinical Science, Intervention & Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden

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A Romano Department of Obstetrics and Gynaecology, Maastricht University Medical Centre+, Maastricht, The Netherlands
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands

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mL ethanol was added dropwise over the course of 1 h. After 1 h, the round-bottomed flask was the fitted with a condenser, transferred to an oil bath, and refluxed for 2 h. After cooling, an orange precipitate was allowed to develop in the fridge over

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Hiroshi Kobayashi Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Japan
Department of Gynecology, Ms.Clinic MayOne, Kashihara, Japan

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Hiroshi Shigetomi Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Japan
Aska Ladies Clinic, Nara, Japan

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Shogo Imanaka Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Japan
Department of Gynecology, Ms.Clinic MayOne, Kashihara, Japan

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defense in endometriosis and its malignant transformation . Oxidative Medicine and Cellular Longevity 2015 848595 . ( https://doi.org/10.1155/2015/848595 ) 26185594 Jana SK Dutta M Joshi M Srivastava S Chakravarty B Chaudhury K 2013 1H NMR

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Murong Xu Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Mingpeng Zhao Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Raymond Hang Wun Li Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China

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Zhixiu Lin Hong Kong Institute of Integrative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China

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Jacqueline Pui Wah Chung Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin Chiu Li Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin-Lap Lee School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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David Yiu Leung Chan Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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attaching to the vaginal epithelial surface, the gel starts releasing N-9 slowly at a stable pace, and the protection has been claimed to last for as long as 24 h ( Sangi-Haghpeykar et al. 1996 ). Table 1 Spermicide products containing N-9. Various

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Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

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Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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30 min to 1 h, depending on the quality of the original sample, to achieve an adequate recovery of spermatozoa. The DGC technique was conducted at room temperature but involved exposure of friable human spermatozoa to the physical shearing forces

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