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The connection between vaginal microbiota-metabolite profiles and preterm delivery (PTD, birth <37 weeks’ gestation) has been a topical subject. Previous research has focused on specific metabolites using proton NMR spectroscopy ( 1 H-NMR
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and lower motility populations by 1 H-NMR ( Reynolds et al. 2017 a ). This showed that the integrals from both the choline and lactate/lipid regions of the NMR spectrum were significantly higher in sperm with poorer motility compared to those with
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were determined by liquid chromatography-mass spectrometry and proton NMR spectroscopy ( 1 H-NMR). Figure 1 Lactobacillus crispatus inhibits growth of Gardnerella vaginalis . (A) Gram staining showing zone of inhibition (ZoI, Gram-positive rods
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Department of Internal Medicine, Ponce Health Sciences University, Ponce, Puerto Rico, USA
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transformation in primary and metastatic ovarian cancer . PLoS ONE 6 e19963. ( https://doi.org/10.1371/journal.pone.0019963 ) Ghazi N Arjmand M Akbari Z Mellat AO Saheb-Kashaf H Zamani Z 2016 1H NMR-based metabolomics approaches as non
GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands
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Department of Clinical Genetics, Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands
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GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands
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GROW School for Oncology and Reproduction, Maastricht University, Universiteitssingel, Maastricht, The Netherlands
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Department of Clinical Genetics, Maastricht University Medical Centre (MUMC+), Maastricht, The Netherlands
Division of Obstetrics and Gynaecology, Department of Clinical Science, Intervention & Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden
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mL ethanol was added dropwise over the course of 1 h. After 1 h, the round-bottomed flask was the fitted with a condenser, transferred to an oil bath, and refluxed for 2 h. After cooling, an orange precipitate was allowed to develop in the fridge over
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Aska Ladies Clinic, Nara, Japan
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Department of Gynecology, Ms.Clinic MayOne, Kashihara, Japan
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defense in endometriosis and its malignant transformation . Oxidative Medicine and Cellular Longevity 2015 848595 . ( https://doi.org/10.1155/2015/848595 ) 26185594 Jana SK Dutta M Joshi M Srivastava S Chakravarty B Chaudhury K 2013 1H NMR
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attaching to the vaginal epithelial surface, the gel starts releasing N-9 slowly at a stable pace, and the protection has been claimed to last for as long as 24 h ( Sangi-Haghpeykar et al. 1996 ). Table 1 Spermicide products containing N-9. Various
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Monash IVF Group, Sydney, NSW, Australia
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30 min to 1 h, depending on the quality of the original sample, to achieve an adequate recovery of spermatozoa. The DGC technique was conducted at room temperature but involved exposure of friable human spermatozoa to the physical shearing forces