Search Results

You are looking at 1 - 10 of 23 items for :

  • chemotherapy x
Clear All
Caroline M Allen Biomedical Sciences, University of Edinburgh, Edinburgh, UK

Search for other papers by Caroline M Allen in
Google Scholar
PubMed
Close
,
Federica Lopes MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

Search for other papers by Federica Lopes in
Google Scholar
PubMed
Close
,
Rod T Mitchell MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK

Search for other papers by Rod T Mitchell in
Google Scholar
PubMed
Close
, and
Norah Spears Biomedical Sciences, University of Edinburgh, Edinburgh, UK

Search for other papers by Norah Spears in
Google Scholar
PubMed
Close

Children undergoing chemotherapy to treat childhood cancers are at risk of infertility in adulthood due to drug cytotoxicity ( Allen et al. 2018 ). The development of fertility preservation strategies is therefore vital, particularly for

Open access
Lauren R Alesi Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

Search for other papers by Lauren R Alesi in
Google Scholar
PubMed
Close
,
Quynh-Nhu Nguyen Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia
Paediatric Integrated Cancer Service, VIC, Australia

Search for other papers by Quynh-Nhu Nguyen in
Google Scholar
PubMed
Close
,
Jessica M Stringer Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

Search for other papers by Jessica M Stringer in
Google Scholar
PubMed
Close
,
Amy L Winship Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

Search for other papers by Amy L Winship in
Google Scholar
PubMed
Close
, and
Karla J Hutt Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia

Search for other papers by Karla J Hutt in
Google Scholar
PubMed
Close

prioritised. Female fertility is governed by the quantity and quality of oocytes, which are stored in the ovary within primordial follicles. Cytotoxic chemotherapy, in addition to radiotherapy and surgery, has long been the mainstay of many cancer treatment

Open access
Charvi Kanodia Edinburgh Medical School, University of Edinburgh, UK

Search for other papers by Charvi Kanodia in
Google Scholar
PubMed
Close
,
Michael P Rimmer MRC Centre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, UK

Search for other papers by Michael P Rimmer in
Google Scholar
PubMed
Close
,
Kathleen Duffin Biomedical Sciences, University of Edinburgh, UK

Search for other papers by Kathleen Duffin in
Google Scholar
PubMed
Close
, and
Rod T Mitchell MRC Centre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, UK

Search for other papers by Rod T Mitchell in
Google Scholar
PubMed
Close

Chemotherapy exposure may reduce fertility in males. Adult men may cryopreserve sperm prior to commencing cancer therapy; however, for pre-pubertal males who do not produce sperm, fertility preservation remains experimental. At present, no human

Open access
Roseanne Rosario MRC Centre for Reproductive Health, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, UK

Search for other papers by Roseanne Rosario in
Google Scholar
PubMed
Close
,
Wanyuan Cui Department of Medical Oncology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
The Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia

Search for other papers by Wanyuan Cui in
Google Scholar
PubMed
Close
, and
Richard A Anderson MRC Centre for Reproductive Health, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, UK

Search for other papers by Richard A Anderson in
Google Scholar
PubMed
Close

lack of knowledge of their impact on reproductive function. For many years, the mainstays of cancer treatment have been surgery, radiotherapy, and chemotherapy, with cytotoxic chemotherapy being the main systemic approach to cancer drug therapy

Open access
Valentina Pampanini V Pampanini, Bambino Gesu Pediatric Hospital, Roma, 00165, Italy

Search for other papers by Valentina Pampanini in
Google Scholar
PubMed
Close
,
Lena Sahlin L Sahlin, Women's and Children's Health, Karolinska University Hospital, Stockholm, Sweden

Search for other papers by Lena Sahlin in
Google Scholar
PubMed
Close
,
Elina Holopainen E Holopainen, Obstetrics and Gynecology, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Elina Holopainen in
Google Scholar
PubMed
Close
,
Mervi Taskinen M Taskinen, Division of Hematology-Oncology and Stem Cell Transplantation, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Mervi Taskinen in
Google Scholar
PubMed
Close
,
Mikael Koskela M Koskela, Obstetrics and Gynecology, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Mikael Koskela in
Google Scholar
PubMed
Close
,
Kim Vettenranta K Vettenranta , Division of Hematology-Oncology and Stem Cell Transplantation, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Kim Vettenranta in
Google Scholar
PubMed
Close
,
Jaana Vettenranta J Vettenranta, Division of Hematology-Oncology and Stem Cell Transplantation, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Jaana Vettenranta in
Google Scholar
PubMed
Close
,
Tiina Laine T Laine, Pediatric Research Center, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Tiina Laine in
Google Scholar
PubMed
Close
,
Claudia Anderson C Anderson, Pediatric Research Center, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Claudia Anderson in
Google Scholar
PubMed
Close
, and
Kirsi Jahnukainen K Jahnukainen, Division of Hematology-Oncology and Stem Cell Transplantation, Helsinki University Central Hospital, Helsinki, Finland

Search for other papers by Kirsi Jahnukainen in
Google Scholar
PubMed
Close

The aim of this study was to identify pitfalls in ovarian tissue cryopreservation protocol from referral to surgical procedure and to analyze factors associated with chemotherapy exposure of the cryopreserved tissue and decreased ovarian function in a cohort of young girls at high risk of infertility.

The study population comprised 200 girls eligible for ovarian tissue cryopreservation between 2002 and 2020 at the Children's Hospital of the University Central Hospital of Helsinki (Finland). Analyses included evaluation of the proportion of patients who underwent ovarian tissue cryopreservation, factors associated with patient selection and timing of ovarian tissue cryopreservation, and ovarian function during long-term follow-up in relation to oncological treatments. Lack of counselling was identified as the major reason for not receiving ovarian tissue cryopreservation. A longer interval from scheduling gonadotoxic therapy to cryopreservation correlated with a higher exposure to alkylating agents of the ovarian tissue. The long-term ovarian function was mainly influenced by age at the time of gonadotoxic treatment. Current selection criteria for ovarian tissue cryopreservation should be implemented in order to stratify patients at risk of infertility and timely identify those at higher risk, especially in relation to age and pubertal stage. Efforts to increase healthcare providers’ awareness and facilitate guided timing in relation to the treatment protocols are needed to guarantee early access to ovarian tissue cryopreservation for all patients at high risk of infertility.

Open access
Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Alena J Hungerford in
Google Scholar
PubMed
Close
,
Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

Search for other papers by Hassan W Bakos in
Google Scholar
PubMed
Close
, and
Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

Search for other papers by Robert J Aitken in
Google Scholar
PubMed
Close

Graphical abstract

Abstract

Sperm cryopreservation is a valuable tool for the long-term preservation of male fertility. Thus, determining the optimal technique for isolating spermatozoa post-thaw is vital to ensure recovery of the highest quality spermatozoa with minimal iatrogenic damage. This not only enhances the chances of successful conception but also reduces the risk of genetic damage in the embryo. To address this issue, human semen samples were cryopreserved using a slow freezing protocol and Quinn's Advantage™ Sperm Freeze medium. The samples were subsequently thawed and subjected to three types of sperm isolation procedures: direct swim-up, density gradient centrifugation, and electrophoretic separation using the Felix™ device. Cryopreservation led to the anticipated loss of sperm motility and vitality in association with increases in lipid peroxidation and DNA damage. Following sperm selection, all three isolation techniques resulted in an increase in sperm motility which was particularly evident with the swim-up and Felix™ procedures. The latter also significantly improved sperm vitality. There were no differences between sperm separation techniques with respect to morphology, and mitochondrial reactive oxygen species generation remained essentially unchanged when cell vitality was taken into account. By contrast, major differences were observed in DNA integrity and lipid aldehyde formation, where Felix™ isolated cells exhibiting significantly less DNA damage than the other isolation procedures as well as lower levels of 4-hydroxynonenal formation. Electrophoretic sperm isolation, therefore, offers significant advantages over alternative separation strategies, in terms of the quality of the gametes isolated and the time taken to achieve the isolation.

Lay Summary

Long-term storage of sperm is vital to assisted reproductive technology because it permits the preservation of fertility that might be compromised as a result of factors such as chemotherapy or vasectomy. This goal can be achieved via cryopreservation – the freezing of cells to −196°C. When the sperm are subsequently required for conception, they must be carefully separated from the cryopreservation medium in a manner that maximizes the chances of successful conception and minimizes the risk of genetic defects in the offspring. In this paper, three isolation techniques were compared for their ability to separate ideal sperm from semen and media following cryopreservation. It was found that cryopreservation led to lower levels of motility and vitality and created higher levels of DNA and cell membrane damage. Of the three techniques compared, only cells separated on the basis of their size and electric charge (electrophoretic isolation) exhibited significantly lower levels of DNA fragmentation.

Open access
Rod T Mitchell MRC Centre for Reproductive Health, The Queen’s Medical Research Institute, The University of Edinburgh, Edinburgh, UK
Department of Paediatric Endocrinology, Royal Hospital for Children and Young People, Edinburgh, UK

Search for other papers by Rod T Mitchell in
Google Scholar
PubMed
Close
and
Suzannah A Williams Nuffield Department of Women’s and Reproductive Health, Women’s Centre, John Radcliffe Hospital, University of Oxford, Oxford, UK

Search for other papers by Suzannah A Williams in
Google Scholar
PubMed
Close

offered to all men due to receive any treatment with a risk of impaired fertility (e.g. chemotherapy or radiotherapy). But what about when spermatogenesis is impaired? In this series, Dr Sarah Martins Da Silva will discuss the options and practicalities in

Open access
Briet D Bjarkadottir Nuffield Department of Women’s and Reproductive Health, University of Oxford, Oxford, UK

Search for other papers by Briet D Bjarkadottir in
Google Scholar
PubMed
Close
,
Charlotte A Walker Nuffield Department of Women’s and Reproductive Health, University of Oxford, Oxford, UK

Search for other papers by Charlotte A Walker in
Google Scholar
PubMed
Close
,
Muhammad Fatum Nuffield Department of Women’s and Reproductive Health, University of Oxford, Oxford, UK
Department of Paediatric Oncology and Haematology, Oxford University Hospitals NHS Foundation Trust, Oxford, UK

Search for other papers by Muhammad Fatum in
Google Scholar
PubMed
Close
,
Sheila Lane Oxford Fertility, Institute of Reproductive Sciences, Oxford, UK

Search for other papers by Sheila Lane in
Google Scholar
PubMed
Close
, and
Suzannah A Williams Nuffield Department of Women’s and Reproductive Health, University of Oxford, Oxford, UK

Search for other papers by Suzannah A Williams in
Google Scholar
PubMed
Close

criteria included ovarian cancer and prior chemotherapy or radiation treatment. As part of the consent process, permission to use tissue in research had been obtained. Cortical strips were cryopreserved using slow freezing by the Oxford Cell and Tissue

Open access

Oogonial stem cells: the unexpected superheroes

The time has come for stem cell technologies to be used in fertility studies

Rita L Sousa Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium

Search for other papers by Rita L Sousa in
Google Scholar
PubMed
Close

technology is successful, it has the potential to be used to preserve fertility for women before they undergo chemotherapy or radiation treatments or to restore fertility in women who already underwent treatment, are menopausal or have POF. We also aim to

Open access
Synneva Hagen-Lillevik Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA
Department of Nutrition and Integrative Physiology, University of Utah College of Health, Salt Lake City, Utah, USA

Search for other papers by Synneva Hagen-Lillevik in
Google Scholar
PubMed
Close
,
John S Rushing Divisions of Reproductive Sciences, Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Colorado Denver (AMC), Aurora, Colorado, USA

Search for other papers by John S Rushing in
Google Scholar
PubMed
Close
,
Leslie Appiah Division of General Obstetrics and Gynecology, Department of Obstetrics and Gynecology, University of Colorado Denver (AMC), Anschutz Outpatient Pavilion, Aurora, Colorado, USA

Search for other papers by Leslie Appiah in
Google Scholar
PubMed
Close
,
Nicola Longo Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA
Department of Nutrition and Integrative Physiology, University of Utah College of Health, Salt Lake City, Utah, USA

Search for other papers by Nicola Longo in
Google Scholar
PubMed
Close
,
Ashley Andrews Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA

Search for other papers by Ashley Andrews in
Google Scholar
PubMed
Close
,
Kent Lai Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA
Department of Nutrition and Integrative Physiology, University of Utah College of Health, Salt Lake City, Utah, USA

Search for other papers by Kent Lai in
Google Scholar
PubMed
Close
, and
Joshua Johnson Divisions of Reproductive Sciences, Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Colorado Denver (AMC), Aurora, Colorado, USA

Search for other papers by Joshua Johnson in
Google Scholar
PubMed
Close

). Additionally, stimulation of AKT phosphorylation and inhibition of PTEN in cortical fragments of ovaries from human patients receiving chemotherapy were able to activate primordial follicles and produced two live human births ( Kawamura et al. 2013 , Suzuki

Open access