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Emmanuel Amabebe Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Steven Reynolds Academic Unit of Radiology, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK

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Dilly O C Anumba Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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delivery (term = 250 and preterm = 50), we examined its potential to identify metabolic features associated with spontaneous PTD (sPTD) and microbiota community state types (CST). CSTs were determined for 83 of these samples by sequencing the V1–V3 region

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Emmanuel Amabebe Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Dilly O Anumba Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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. 2017 ). The vaginal microbiota at late gestation is similar to that of a non-pregnant (non-menstruating and bacterial vaginosis, BV-negative) state characterised by a decrease in α-diversity (species richness – within individual diversity or amount of

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Mary Ann Ottinger Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA

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Shailaja Mani Department of Molecular & Cellular Biology and Neuroscience, Baylor College of Medicine, Houston, Texas, USA

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Sonja Krüger Ezemvelo KwaZulu-Natal Wildlife, Scientific Services, Queen Elizabeth Park, 1 Peter Brown Drive, Pietermaritzburg, South Africa
Centre for Functional Biodiversity, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa

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Brent Coverdale Ezemvelo KwaZulu-Natal Wildlife, Scientific Services, Queen Elizabeth Park, 1 Peter Brown Drive, Pietermaritzburg, South Africa

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Sandi Willows-Munro Centre for Functional Biodiversity, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa

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Leigh Combrink School of Natural Resources and the Environment, University of Arizona, Tucson, Arizona, USA

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and establishment of the gut microbiome in arctic shorebirds . FEMS Microbiology Ecology 93 . ( https://doi.org/10.1093/femsec/fix142 ) Grond K Sandercock BK Jumpponen A & Zeglin LH 2018 The avian gut microbiota: community, physiology and

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Caitriona Brennan Department of Pediatrics, University of California San Diego, La Jolla, California, USA
Division of Biological Sciences, University of California San Diego, La Jolla, California, USA

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Kristina Chan Department of Pediatrics, University of California San Diego, La Jolla, California, USA
Department of Bioengineering, University of California, San Diego, La Jolla, California, USA

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Tanya Kumar Medical Scientist Training Program, University of California San Diego, La Jolla, California, USA

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Erica Maissy Division of Gastroenterology, University of California San Diego, La Jolla, California, USA
Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California, USA

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Linda Brubaker Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Diego, La Jolla, California, USA

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Marisol I Dothard Department of Pediatrics, University of California San Diego, La Jolla, California, USA
Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California, USA

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Jack A Gilbert Department of Pediatrics, University of California San Diego, La Jolla, California, USA
Center for Microbiome Innovation, University of California San Diego, La Jolla, California, USA

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Katharine E Gilbert Department of Pediatrics, University of California San Diego, La Jolla, California, USA

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Amanda L Lewis Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Diego, La Jolla, California, USA

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Varykina G Thackray Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Diego, La Jolla, California, USA
Center for Microbiome Innovation, University of California San Diego, La Jolla, California, USA

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Amir Zarrinpar Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
Medical Scientist Training Program, University of California San Diego, La Jolla, California, USA
Division of Gastroenterology, University of California San Diego, La Jolla, California, USA
Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Diego, La Jolla, California, USA
Center for Microbiome Innovation, University of California San Diego, La Jolla, California, USA
Jennifer Moreno Department of Veterans Affairs Medical Center, La Jolla, California, USA
Institute of Diabetes and Metabolic Health, University of California San Diego, La Jolla, California, USA

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Rob Knight Department of Pediatrics, University of California San Diego, La Jolla, California, USA
Department of Bioengineering, University of California, San Diego, La Jolla, California, USA
Center for Microbiome Innovation, University of California San Diego, La Jolla, California, USA
Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California, USA
Halıcıoğlu Data Science Institute, University of California San Diego, La Jolla, California, USA

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-specific disease. The vaginal microbiota’s community composition has been classified into five unique community state types (CSTs), several of which are dominated by lactic acid-producing bacteria belonging to the Lactobacillus genus ( Ravel et al. 2011 , Kwon

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Landon K Eldridge Department of Animal Science, Texas A&M University, College Station, Texas, USA
Department of Animal Science, University of Wyoming, Laramie, Wyoming, USA

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Dallas R Soffa Department of Animal Science, Texas A&M University, College Station, Texas, USA

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Kyle J Hickman-Brown Department of Animal Science, Texas A&M University, College Station, Texas, USA

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Brooke E McAnally Department of Animal Science, Texas A&M University, College Station, Texas, USA

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Molly S Smith Department of Animal Science, Texas A&M University, College Station, Texas, USA

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Jeffrey G Wiegert Department of Animal Science, Texas A&M University, College Station, Texas, USA

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Rebecca K Poole Department of Animal Science, Texas A&M University, College Station, Texas, USA

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piglets with lower weight gain (i.e. decreased milk consumption). Interestingly, administering an in-feed probiotic to sows during gestation altered the dam GIT microbiome and the microbiota of the piglets born, suggesting an apparent link between the

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Emmanuel Amabebe Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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Henry Ogidi Department of Obstetrics and Gynaecology, Glan Clwyd Hospital North Wales, Gwynedd, UK

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Dilly O Anumba Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

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in altering MMP activity for both labour and CIN/cancer The normal vaginal microbiota is dominated by lactobacilli and exhibits low immunostimulatory capacity in pregnant and non-pregnant state ( Amabebe & Anumba 2018 , 2020 ). An altered vaginal

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Camila N Ortiz Department of Basic Sciences, Ponce Health Sciences University, Ponce Research Institute, Ponce, Puerto Rico, USA

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Annelyn Torres-Reverón DHR Health Institute for Research and Development, Edinburg, Texas, USA

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Caroline B Appleyard Department of Basic Sciences, Ponce Health Sciences University, Ponce Research Institute, Ponce, Puerto Rico, USA
Department of Internal Medicine, Ponce Health Sciences University, Ponce, Puerto Rico, USA

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. 2018 a, b ). Metabolomics is the study of small molecules within biofluids or tissues and gives information about the physiological state of a cell tissue or biofluid ( EVAR Workgroup et al. 2011 , Goulidelmos et al. 2020 ). Different types of

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Rhiannon L Bolton Nature’s SAFE, Chapel Field Stud, Ash Lane, Whitchurch, Shropshire, UK

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Andrew Mooney Dublin Zoo, Phoenix Park, Dublin 8, Ireland

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Matt T Pettit Nature’s SAFE, Chapel Field Stud, Ash Lane, Whitchurch, Shropshire, UK
IMT International Limited, Tattenhall, Chester, UK

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Anthony E Bolton Nature’s SAFE, Chapel Field Stud, Ash Lane, Whitchurch, Shropshire, UK

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Lucy Morgan Gemini Genetics, Chapel Field Stud, Ash Lane, Whitchurch, UK

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Gabby J Drake Chester Zoo, Upton-by-Chester, UK

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Ruth Appeltant Nuffield Department of Women’s and Reproductive Health, University of Oxford, Women’s Centre, Level 3, John Radcliffe Hospital, Oxford, UK

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Susan L Walker Nature’s SAFE, Chapel Field Stud, Ash Lane, Whitchurch, Shropshire, UK
Chester Zoo, Upton-by-Chester, UK

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James D Gillis South-East Zoo Alliance for Reproduction & Conservation, Yulee, Florida, USA

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Christina Hvilsom Copenhagen Zoo, Frederiksberg, Denmark

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found in isolated or inaccessible locations, making sample acquisition and transport both difficult and expensive ( Ryder & Onuma 2018 , Houck 2019 ). However, the global zoo and aquarium community represent a unique resource for samples, either through

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