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Steven Reynolds Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Sarah J Calvert Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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Stephen J Walters School of Health Related Research, University of Sheffield, Regent Court, Sheffield, UK

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Martyn N Paley Imaging Sciences, Department of Immunity, Infection and Cardiovascular Disease, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

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Allan A Pacey Department of Oncology and Metabolism, University of Sheffield, The Jessop Wing, Tree Root Walk, Sheffield, UK

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Introduction Asthenozoospermia is defined as <32% progressive sperm motility in semen, and while it can be readily identified at semen analysis, the underlying causes are not known ( WHO 2010 ). A number of studies have examined

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0101
Volume/Issue:
Volume 3: Issue 2
Open access
Lisa Windhofer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Auke Boersma Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Maik Dahlhoff Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Thomas Rülicke Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

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Kerstin E Auer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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the expansion of a line ( Behringer et al. 2014 , Taft 2017 ). In addition, sperm collection is performed to investigate sperm quality traits like sperm motility or swimming velocity. These traits can work as a proxy to estimate male fertility in

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0017
Volume/Issue:
Volume 4: Issue 2
Open access
Murong Xu Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Mingpeng Zhao Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Raymond Hang Wun Li Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China

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Zhixiu Lin Hong Kong Institute of Integrative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China

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Jacqueline Pui Wah Chung Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin Chiu Li Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin-Lap Lee School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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David Yiu Leung Chan Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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clinical trials studying the effects of N-9 focused on the sperm structure and functions. Sperm motility determines the ability of sperm to move toward the egg through the female reproductive tract. It is considered as the most important parameter to

Article Type:
Review Article
DOI:
https://doi.org/10.1530/RAF-21-0024
Volume/Issue:
Volume 3: Issue 1
Open access
Ola S Davis Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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Vivien B Truong Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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Katie D Hickey Department of Research and Development, Semex Alliance, Guelph, Ontario, Canada

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Laura A Favetta Reproductive Health and Biotechnology Lab, Department of Biomedical Sciences, OVC, University of Guelph, Guelph, Ontario, Canada

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(in the case of ART use), or in the female reproductive tract ( Vitku et al. 2016 ). Sperm require both motility and capacitation abilities in order to fertilize an egg. In 2015, Lukacova et al. reported decreased bovine sperm motility after in

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0018
Volume/Issue:
Volume 4: Issue 4
Open access
Jennifer B Nagashima Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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Marcia de Almeida Monteiro Melo Ferraz Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Gene Center, Ludwig-Maximilians University, Munich, Bayern, Germany

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Sarah H Kamen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA
Department of Biological and Environmental Sciences, Longwood University, Farmville, Virginia, USA

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Nucharin Songsasen Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, Virginia, USA

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( Lockyear et al. 2009 ), and cryoprotectant and post-thaw culture temperatures ( Franklin et al. 2018 ), have improved survival and motility post-thaw. However, sperm motility/viability has been shown to drop precipitously during post-thaw incubation in

Article Type:
Research Article
Collections:
Wildlife
DOI:
https://doi.org/10.1530/RAF-20-0042
Volume/Issue:
Volume 1: Issue 1
Open access
Fatima Kazue Okada Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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Rhayza Roberta Andretta Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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Deborah Montagnini Spaine Human Reproduction Section, Division of Urology, Department of Surgery, Federal University of São Paulo, R. Borges Lagoa, São Paulo, São Paulo, Brazil

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activity ( Shen et al. 2019 ), and acrosome integrity ( Levitas et al. 2006 , Shen et al. 2019 ). Mayorga-Torres et al. (2015) have shown that a longer EA is associated with increases in semen volume and sperm count. Sperm motility, viability

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-20-0018
Volume/Issue:
Volume 1: Issue 1
Open access
Maria Handzhiyska Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Rumiana Ganeva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Dimitar Parvanov Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Margarita Ruseva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Petar Eftimov Department of Cell and Developmental Biology, Faculty of Biology, SU St. Climent Ohridski, Sofia, Bulgaria

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Vilyana Georgieva Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Denitsa Velikova Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Georgi Stamenov Department of Obstetrics and Gynecology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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application of this method selects spermatozoa with better motility ( AL-Marayaty et al. 2017 ), morphology ( Heidari et al. 2018 ), and lower sperm DNA fragmentation rates compared to native sperm ( Younglai et al. 2001 , Parmegiani et al. 2010

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0052
Volume/Issue:
Volume 5: Issue 2
Open access
Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

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Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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in a wide variety of ways, including reduced sperm number, lowered sperm motility, abnormal sperm morphology, impaired capacitation and sperm-egg recognition, lowered vitality, DNA damage, and oxidative stress ( Zini & Libman 2006 , Aitken & Roman

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-22-0133
Volume/Issue:
Volume 4: Issue 2
Open access
Clara Malo Camel Reproduction Centre, Dubai, UAE

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Sara Carracedo SPQI S.A.S, Lille, France

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Maryse Delehedde SPQI S.A.S, Lille, France

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Nicolas Sergeant SPQI S.A.S, Lille, France
University of Lille, CHRU, INSERM UMRS 1172, Lille, France

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Julian Alexandra Skidmore Camel Reproduction Centre, Dubai, UAE

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countries, showing greater values from December to February and the least in June and July. These anatomical changes are associated with sperm parameter variations including greater volume, motility, velocities and viability during the rutting months ( Deen

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0055
Volume/Issue:
Volume 2: Issue 4
Open access
Shen Chuen Khaw NHS Tayside, Ninewells Hospital, Dundee, UK

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Zhen Zhe Wong International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Federal Territory of Kuala Lumpur, Malaysia

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Richard Anderson MRC Centre for Reproductive Health, Queen’s Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK

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Sarah Martins da Silva Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK

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mechanism(s) of OS in reducing sperm quality is unknown, it is widely acknowledged that depleted intracellular ATP levels, insufficient axoneme phosphorylation and lipid peroxidation of the cell membrane manifests as poor motility and sperm dysfunction

Article Type:
Research Article
Collections:
Clinical research and reviews
DOI:
https://doi.org/10.1530/RAF-20-0037
Volume/Issue:
Volume 1: Issue 1
Open access