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Rumiana Ganeva Research Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Dimitar Parvanov Research Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Denitsa Velikova Andrology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Magdalena Vasileva Embryology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Kristina Nikolova Embryology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Georgi Stamenov Obsterics and Gynecology Department, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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devices ( Quinn et al. 2018 , Parrella et al. 2019 ), and motile sperm organelle morphology examination ( Bartoov et al. 2003 , Garolla et al. 2014 ). However, following the natural sperm selection process, another non-invasive approach has

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0041
Volume/Issue:
Volume 2: Issue 3
Open access
Maria Handzhiyska Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Rumiana Ganeva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Dimitar Parvanov Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Margarita Ruseva Department of Research, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Petar Eftimov Department of Cell and Developmental Biology, Faculty of Biology, SU St. Climent Ohridski, Sofia, Bulgaria

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Vilyana Georgieva Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Denitsa Velikova Department of Andrology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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Georgi Stamenov Department of Obstetrics and Gynecology, Nadezhda Women’s Health Hospital, Sofia, Bulgaria

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al. 1997 , Kruger & Coetzee 1999 , Van Waart et al. 2001 , Jin et al. 2015 , Dcunha et al. 2022 ). One of the most commonly applied techniques for sperm selection is swim-up ( Volpes et al. 2016 ). Previous studies have proven that the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0052
Volume/Issue:
Volume 5: Issue 2
Open access
Dimitra Apostolia Androni Assisted Conception Service, Glasgow Royal Infirmary, NHS Greater Glasgow and Clyde,Glasgow, UK

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Sophie Dodds Semovo Ltd, Manchester Fertility, Cheadle, UK

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Mathew Tomlinson Fertility Unit, Nottingham University Hospital, Nottingham, UK
School of Medicine, Division Child Health, Obstetrics and Gynaecology, Queen’s Medical Centre, Nottingham, UK

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Walid E Maalouf School of Medicine, Division Child Health, Obstetrics and Gynaecology, Queen’s Medical Centre, Nottingham, UK

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, different authors have investigated the effect of sperm preparation before freezing as a potentially superior sperm selection approach. However, studies are conflicting and lack consistency especially in relation to: The number of pre-freeze and post

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-20-0041
Volume/Issue:
Volume 2: Issue 1
Open access
Alena J Hungerford Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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Hassan W Bakos Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia
Monash IVF Group, Sydney, NSW, Australia

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Robert J Aitken Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia

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, level of mitochondrial ROS generation, and DNA damage. Not surprisingly, all of the sperm selection techniques generated a reduced number of spermatozoa compared with the unselected control ( P <0.001). With respect to the individual sperm isolation

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-22-0133
Volume/Issue:
Volume 4: Issue 2
Open access
Darren J X Chow Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia
Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia
Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia

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Philip Wijesinghe SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom

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Kishan Dholakia Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia
SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom
School of Biological Sciences, The University of Adelaide, Adelaide, Australia
Department of Physics, College of Science, Yonsei University, Seoul, South Korea

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Kylie R Dunning Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia
Australian Research Council Centre of Excellence for Nanoscale Biophotonics, The University of Adelaide, Adelaide, Australia
Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia

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cytoplasmic characteristics. In terms of sperm, morphology, concentration, and motility are known factors that are directly correlated with IVF success. However, the selection is prone to a high degree of variation between operators. AI can be transformative

Article Type:
Discussion
DOI:
https://doi.org/10.1530/RAF-21-0043
Volume/Issue:
Volume 2: Issue 3
Open access
Alex Polyakov Faculty of Medicine and Health Sciences, University of Melbourne, Melbourne, Australia
Reproductive Biology Unit, Royal Women’s Hospital, Melbourne, Australia

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Genia Rozen Faculty of Medicine and Health Sciences, University of Melbourne, Melbourne, Australia
Reproductive Biology Unit, Royal Women’s Hospital, Melbourne, Australia

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same-sex couples, the involvement of a sperm donor is required for posthumous reproduction. This introduces issues related to donor selection, legal parentage, and rights of non-biological parents. Furthermore, posthumous conception with the deceased

Article Type:
Discussion
DOI:
https://doi.org/10.1530/RAF-23-0072
Volume/Issue:
Volume 4: Issue 4
Open access
Lisa Windhofer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Auke Boersma Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Maik Dahlhoff Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Thomas Rülicke Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

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Kerstin E Auer Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria

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Introduction Sperm collection in mice is frequently applied in laboratory rodent facilities, and it is especially important in assisted reproduction. Spermatozoa are collected to cryopreserve valuable mouse strains ( Nakagata 2000 , Wilson

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-23-0017
Volume/Issue:
Volume 4: Issue 2
Open access
Fabien Joao Fertilys Fertility Center, Laval, Quebec, Canada

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Cyntia Duval Fertilys Fertility Center, Laval, Quebec, Canada

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Marie-Claire Bélanger Fertilys Fertility Center, Laval, Quebec, Canada
Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), CHUM Research Center, Montreal, Quebec, Canada

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Julie Lamoureux Fertilys Fertility Center, Laval, Quebec, Canada

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Cheng Wei Xiao Fertilys Fertility Center, Laval, Quebec, Canada

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Senem Ates Fertilys Fertility Center, Laval, Quebec, Canada

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Moncef Benkhalifa Fertilys Fertility Center, Laval, Quebec, Canada
Université Picardie Jules Verne, Médecine et Biologie de la Reproduction et Laboratoire PERITOX, CBH-CHU Amiens Picardie, Amiens, France

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Pierre Miron Fertilys Fertility Center, Laval, Quebec, Canada

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be isolated from the seminal plasma in order to perform intra uterine insemination or in vitro fertilization, whether by a migration or filtration method or by the selection of mature sperm ( Mehta & Sigman 2014 ). Clinicians need to know if there

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-21-0005
Volume/Issue:
Volume 3: Issue 2
Open access
Murong Xu Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Mingpeng Zhao Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Raymond Hang Wun Li Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China

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Zhixiu Lin Hong Kong Institute of Integrative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Sha Tin, Hong Kong, China

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Jacqueline Pui Wah Chung Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin Chiu Li Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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Tin-Lap Lee School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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David Yiu Leung Chan Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

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used to evaluate the publication bias of the studies. The symmetric shape indicated the precision of data and a small likelihood of publication bias. Results Meta-analysis of the effects of N-9 on sperm motility Study selection There

Article Type:
Review Article
DOI:
https://doi.org/10.1530/RAF-21-0024
Volume/Issue:
Volume 3: Issue 1
Open access
Yu-Fei Yang College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China
Ningbo Jiangshan High School, Ningbo, Zhejiang, PR China

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Jia-Hao Wu College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China

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Run-Lan Lin College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China

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Shang-Jun Yin College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China

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Guo-Ying Qian College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China

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Wei Wang College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China

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Yong-Doo Park College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, PR China
Skin Diseases Research Center, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, PR China
Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, PR China

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Introduction The process of sperm cell development is complex and refers to the process of spermatogonial cells transforming into sperm over a long time within the boundary of seminiferous tubules of the testis. This process is primarily

Article Type:
Research Article
DOI:
https://doi.org/10.1530/RAF-22-0117
Volume/Issue:
Volume 4: Issue 1
Open access