Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

in Reproduction and Fertility
View More View Less
  • 1 I Al-Saleh, Environmental Health Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 2 S Coskun, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 3 R Al-Rouqi, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 4 T Al-Rajudi, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 5 C Eltabache, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 6 M Abduljabbar, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
  • 7 S Al-Hassan, Department of Obstetrics and Gynecology, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia

Correspondence: Iman Al-Saleh, Email: iman@kfshrc.edu.sa

This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate, biochemical pregnancy, clinical pregnancy, and live birth). We observed an increase in the oxidative stress markers MDA, 8-OHdG, and H2O2 in follicular fluid and seminal plasma, but a decrease in the antioxidant protection markers CAT and TAC. The MDA, 8-OHdG, and H2O2 levels were significantly higher in seminal plasma than in follicular fluid, while TAC, CAT, and TM were higher in follicular fluid (p < 0.001). Although women were nonsmokers, COT levels >50 µg/l were observed in 5.7% (urine) and 1.4% (follicular fluid). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%).

 

     An official journal of

    Society for Reproduction and Fertility

 

Sept 2018 onwards Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 18 18 18
PDF Downloads 18 18 18