The effect of embryo catheter loading technique on pregnancy rate

in Reproduction and Fertility
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  • 1 T Matitashvili, Norfolk , 23503, United States
  • | 2 S Sadek, Norfolk , United States
  • | 3 G Celia, REI, Dominion Fertility, Arlington, United States

Correspondence: Tamar Matitashvili, Email:

Purpose: Embryo transfer is the last and most vital part of the in vitro fertilization treatment process. In recent years embryo transfer protocols have been standardized, but embryo-loading technique did not receive much attention per se. The aim of our study was to compare clinical pregnancy and positive pregnancy test rates between the two different embryo-loading (EL) techniques.

Design: Retrospective cohort study.

Methods: 493 frozen embryo transfer (FET) cycles were included in our retrospective cohort study. From November 2018 until January 2020 “double air bubble” embryo catheter-loading technique was used. Starting from January 2020 until July 2021 lab introduced “medium only” loading-technique. In group A (n=249) loading was done by filling the catheter with 15-20μL of medium and then aspirating 10μL of air followed by 5-10μL of medium containing embryo(s). Adding 10μL of air at the tip of the catheter completed loading. In Group B (n=244) loading was performed by filling the catheter with 15-20μL of medium followed by an additional 5-10μL containing the embryo(s) with no air was aspirated.

Results: No statistical difference was detected between the groups with regard to age, body mass index (BMI), diagnosis, FET cycle characteristics, and quality of embryos transferred (all P>0.05). 493 FETs resulted in 279 positive pregnancy tests and 236 clinical pregnancies. There was no statistical difference between the two groups in clinical pregnancy (47% vs 48.7% P=0.36) and positive pregnancy test rate (55.8% vs 57.3% P= 0.40).

Conclusion: Embryo loading technique did not affect positive pregnancy test or clinical pregnancy rates.


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