Incubation of semen with human follicular fluid improves the antioxidant status and quality of spermatozoa after freezing-thawing
in Reproduction and FertilitySearch for other papers by Monireh Mahmoodi in
Current site
Google Scholar
PubMed
Search for other papers by Elham Shojafar in
Current site
Google Scholar
PubMed
Search for other papers by Maryam Dastjani-Farahani in
Current site
Google Scholar
PubMed
The sperm freezing-thawing procedure is the most commonly used technique in clinics to preserve male fertility before any pathological destruction of the testis. Therefore, most studies are currently focused on optimizing this method to achieve high-quality semen after thawing. During cryopreservation, oxidative stress-induced damage affects sperm structures and decreases their fertility potential. The use of antioxidants in freezing media can protect sperm against oxidative damage. We designed this study to evaluate whether incubation of semen with human follicular fluid, which contains a wide variety of enzymatic and non-enzymatic antioxidants, can prevent the negative effects of freezing-thawing on human spermatozoa. Human semen was divided into three groups 1) the 0-hour group (before freezing), 2) the control group (after freezing-thawing), and 3) the FF group (after freezing with 50% follicular fluid). The sperm motility, viability, integrity of the plasma membrane and DNA, mitochondrial membrane potential, malondialdehyde level, total antioxidant capacity, and catalase activity were assessed in these 3 groups. The findings showed a significant decrease in sperm motility, viability, plasma membrane and DNA integrity, mitochondrial membrane potential, total antioxidant capacity, and catalase activity and a significant increase in malondialdehyde level in the control group compared with the 0-hour group. The FF group displayed a considerable increase in sperm parameters, total antioxidant capacity, catalase activity, and a significant decrease in malondialdehyde level compared with the control group. Follicular fluid can be considered an effective supplement to improve antioxidant indices and sperm parameters during freezing-thawing.
Reproduction and Fertility is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
As an open-access journal, Reproduction and Fertility articles are immediately available to read on publication, without restriction. The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 64 | 64 | 64 |
| PDF Downloads | 76 | 76 | 76 |
Online ISSN: 2633-8386
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519